a mobile line regarded as probably the most widely used in mind cancer research is not the same as the individual that is original from where it had been derived, say Bengt Westermark and colleagues, whom first established the mobile line almost 50 years back. According to the writers, the cell that is publicly available, which has been found in significantly more than 1,700 magazines, seems to be a kind of glioma cell line, likely a glioblastoma, of unknown beginning.
this specific instance of mistaken identity, amongst others, shines a spotlight on misidentification or contamination of mobile lines as a long-standing problem that is quality-control imperils the reproducibility of medical experiments and the overall industry of translational research. A glioma mobile line which has since been made commercially available in repositories, including the United states Type customs Collection (ATCC) in 1968, Westermark and their team founded U87MG. The authors suspect that U87MG is the most cell that is popular created, with approximately 200 studies posted in 2015 alone
Questioning the authenticity associated with the mobile line, Westermark et al. utilized forensic and DNA that is mitochondrial to locate U87MG's origin. This analysis revealed that the foundation regarding the mobile line is different from compared to the tumor that is original pointing to a potential mix-up or cross-contamination. The scientists additionally looked at the signature that is hereditary of, comparing its similarity to a large database of cancer tumors cell lines. Outcomes confirmed that the cells comes from the main system that is stressed. Their findings further stress the importance of authenticating cell lines to produce more consistent research that is scientific. To deal with this matter that is very important authors call on the research community to move far from "classical" cell lines like U87MG in favor of confirmed glioma cellular lines grown in proper conditions, which are far more representative associated with the tumor of origin.
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